ABSTRACT
Objective To develop measles and Japanese encephalitis(JE)combined attenuated live vaccine. Methods The independent development of China′s Shanghai 191 measles vaccine strains and Japanese encephalitis SA14-14-2 vaccine strains were selected to prepare two of monovalent vaccines solution,which were used to conduct the interference test,the ratio test,the screening test of protection agent,the screening test of dilution,and the alternative of the curve of freeze-dried.Results The preparation technology of measles and Japanese encephalitis combined live attenuated vaccine was determined.The batches of the products met the quality requirement of the three kinds of monovalent vaccine in the Pharmacopoeia of the people's Republic of China(edition 2005,volume 3).The cross-interference and increased response were not found in the animal test,and the immune response of mouse was not influenced,which indicated the safety and immunogenicity of with good combined vaccine.Conclusion Measles and Japanese encephalitis combined attenuated live vaccine was developed successfully.